[Primeusers] Cannot run program

arve arve at csc.kth.se
Wed Feb 3 12:43:35 CET 2010


Ja.


Feb 3, 2010 kl. 12:22 PM skrev Bengt Sennblad:

> Har du dubbelkollat att web-versionen (som jag antar att hon har?) har  
> denna option?
> 
> Bengt
> 
> 3 feb 2010 kl. 12.04 skrev arve:
> 
>> I am sorry, the option is "-c 250" (or a number of your choosing,  
>> the default is 50).
>> 
>> 	Lars
>> 
>> 
>> 
>> Feb 3, 2010 kl. 11:58 AM skrev A.Malhotra:
>> 
>>> arve wrote:
>>>> This error has occured before when there are not enough  
>>>> discretization points in the species tree to put the gene nodes  
>>>> on. Try again with an option "-n 250" and see if that helps.
>>>> 
>>>> 	Best regards,
>>>> 	Lars
>>>> 
>>> 
>>> I have tried this and get the message "unknown option "-n"!
>>> 
>>>> 
>>>> Jan 28, 2010 kl. 5:02 PM skrev A.Malhotra:
>>>> 
>>>>> OK, I've sorted this out, I think. I then got a lot of errors  
>>>>> relating
>>>>> to mismatches between the tree file and the gene-species map  
>>>>> file, which
>>>>> I have also corrected. However, the next error has foxed me again:
>>>>> Probability: attempting to divide by zero!
>>>>> 
>>>>> Any ideas what is causing this one?
>>>>> Thanks
>>>>> Anita
>>>>> 
>>>>> arve wrote:
>>>>>> Hi Anita,
>>>>>> The error you get should only occur if the file is not found.  
>>>>>> Have you double checked the name of the file and its location?
>>>>>> 
>>>>>> Yes, there are several parameters that you need to specify at  
>>>>>> this point, for the simple reason that we have not implemented  
>>>>>> their inference yet.
>>>>>> 
>>>>>> We do not support partitioning of data at this point.
>>>>>> 
>>>>>> 	Best regards,
>>>>>> 	Lars
>>>>>> 
>>>>>> Jan 26, 2010 kl. 3:10 PM skrev A.Malhotra:
>>>>>> 
>>>>>>> I am trying to run an analysis using PrimeGSR but get an error  
>>>>>>> message
>>>>>>> saying "could not open sequence file". However, this file is in  
>>>>>>> fasta
>>>>>>> format and I can open it in other programs so there seems to be  
>>>>>>> nothing
>>>>>>> wrong with it.
>>>>>>> 
>>>>>>> primeGSR -o PLA2.out -i 10000000 -t 2000 -w 100 -Su DNA  
>>>>>>> Pi=0.2560 0.2748
>>>>>>> 0.2332 0.236 R=1.5842 4.2435 1.0570 1.5330 4.6701 1.0 -Sn 4 -Sa  
>>>>>>> 0.7564
>>>>>>> -Ed Gamma -Hi vipers.tre nc_nr.fa gs.txt
>>>>>>> 
>>>>>>> I would also appreciate some guidance about setting the model.  
>>>>>>> Normally
>>>>>>> when running a Bayesian phylgenetic analysis (eg in MrBayes) I  
>>>>>>> would not
>>>>>>> set all the parameter values at the beginning but let the  
>>>>>>> Markov chain
>>>>>>> integrate over the uncertainty in these values. However it  
>>>>>>> seems that
>>>>>>> primeGSR requires you to input them. Is this correct and if so,  
>>>>>>> why?
>>>>>>> Finally, I wonder is it is possible to analyse partitioned data  
>>>>>>> in
>>>>>>> primeGSR (eg in MrBayes I can include gaps in the form of a  
>>>>>>> simple-coded
>>>>>>> matrix as a separate partition)?
>>>>>>> Thanks
>>>>>>> Anita
>>>>>>> 
>>> *******************************************************************
>>> Dr Anita Malhotra         	e-mail: a.malhotra at bangor.ac.uk
>>> School of Biological Sciences	Tel(direct line): + 44 1248 383735
>>> College of Natural Sciences	Fax: + 44 1248 371644
>>> Bangor University		
>>> 3rd Floor ECW
>>> Deiniol Road, Bangor		
>>> Gwynedd LL57 2UW
>>> United Kingdom
>>> *******************************************************************
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